Field Flow Fractionation Field flow fraction consists of a wide variety of separation methods for which are used for the purification and characterization of superamolecular compounds like macromolecules, colloids, particles, gels, emulsions and cells. So it has applications especially in the field of biological sciences. It consists of various sub-techniques and separation devices which are used for the separation and characterization of the particles ranging in size from 10-3 to 102 µm. These sub-techniques of the FFF are capable of separating the particles on the basis of their physical properties like charge, mass, size and density. The basic concept of Filed flow fractionation for the first time was given in 1960s by J. Calvin Giddings. Field flow fractionation is considered to be the hybrid of chromatography, electrophoresis and ultracentrifugation all of which are field-driven methods. Like ultracentrifugation is requires a field force and like chromat...