Centrifugation separation techniques The sedimentation rate of the analyte particles in a suspension is dependent upon following properties: · Size of the particle · Medium · External forces applied Stokes’s law is applicable whenever the centrifugal force of ωr 2 is used to carry out sedimentation. Here ω represents the rotational angular velocity and r is representing the distance from the diameter of the analyte particle to the axis of rotation. dr/dt = 2r 2 p (ρ p - ρ m ) ω 2 r/ 9η In this equation dr/dt is the particle’s velocity; η is the viscosity of the medium, r p is the particle’s radius and (ρ p - ρ m ) difference in the density of the particle and the density of the medium. There are various techniques which involve the use of centrifugal force for the separation of particles based upon their rates of sedimentation. These sub...